Authors

Discussion

 Detection And Molecular Analysis of Kinesins in Local Leishmania in Iraq L.Tropica  and L. Donavani 

The Kinesin KIF13 has been identified as a motor protein which has nuclear localisation specifically at the spindle and spindle poles in some kinetoplastids. Five Kinesin-13 members have been shown in T. brucei. TbKIF13-1 is a nuclear protein, TbKIF13-2 and TbKIF13-4 display a flagellar localisation, TbKIF13-3 and TbKIF13-5 are in the cytoplasm. TbKIF13-2 functional analysis in homology proved that the overexpression of TbKIF13-2 reduces flagellum length slightly. Five kinesin-13 family members that belong to the KIF24 subfamily have been identified in the genome of Leishmania major, two of them LmjKIN13-1 and LmjKIN13-2 have been characterised. LmjKIN13-1 has a nuclear localisation specifically at the spindle and spindle poles. Kinesin-13 (MCAK/KIF2) members exhibit a microtubules depolymerising activity responsible for their function in mitosis. The present study focused on the KIF13 in local Leishmania. Sp in Iraq. Firstly L.Tropica which responsible for cutaneous leishmaniasis  and L. donavani responsible for visceral leishmaniasis or kala-azar, the most severe form of leishmaniasis. Initially, PCR technique was used to detect LmxKIF-13 gene, followed by sequence analysis. The study, aimed to identify the function LmKIF-13 in both Leishmania. The kinesin motor protein LmKIF-13 in L.donovani  and L.Tropica can be used as therapeutic and potential vaccine candidate against  leishmaniasis.