Abstract

Cell and gene therapies have rapidly gained popularity but ensuring safety and effectiveness in patients is complex with no room for error. Research, development, and manufacturing laboratories around the world are taking advantage of the precision and convenience of Bio-Rad's Droplet Digital PCR technology as a tool to overcome challenges with existing methods for a range of applications in cell and gene therapy. In contrast, qPCR based QC methods are reliant on standards that are difficult to source and can be variable from batch to catch, adding time and cost. In addition, qPCR based methods are sensitive to PCR inefficiencies like secondary structure and in process impurities, leading to assay failures and expensive assay repeats. Brief overview of main applications and advantages of the ddPCR technology when compared to qPCR.