Poster
3 |
High-Throughput Detergent Screening for Membrane Protein solubilization using nanoliters of sample |
In this work, we demonstrate how Flow Induced Dispersion Analysis (FIDA) is employed for high throughput detergent screening to assess the degree of solubilization and polydispersity of a GFP-tagged membrane protein. The entire screen of 12 detergents takes less than 3 hours and is performed directly in unpurified cell lysates, using only 1,5 uL. The sample tray is kept at 5 ⁰C for the duration of all measurements. We compare the FIDA detergent screening with Fluorescence Size-Exclusion Chromatography (FSEC). The comparison reveals that FIDA decreases the screening time from days to hours and leaves most of the membrane protein available for other experiments.