Abstract

Phage display in conjunction with biopanning is a frequently usedstrategy in the selection process for nanobodies or other expressedbinding proteins with specificity to a target antigen. Here we present a fibreoptics, SPR based approach that combines both fast kinetic characterizationand an efficient selection cycle in a single step. As the assay is based onreal-time kinetics monitoring rather than end point characterization suchas ELISA, it presents potential for selection and characterization onaffinity over avidity.