Authors

D Dacheux¹; MR Miharisoa Rijatiana Ramanantsalama1²; NL Nicolas Landrein²; EC Elina Casas³; BS Bénédicte Salin⁴; CB Corinne Blancard2⁴; MB Mélanie Bonhivers1²; DR Derrick R. Robinson²;  ¹ UMR-5234, Université de Bordeaux, CNRS, Bordeaux INP, Microbiologie Fondamentale et Pathogénicité , F-33000 Bordeaux, France., France;  ² University of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, F-7 33000 Bordeaux, France., France;  ³ University of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, F-7 33000 Bordeaux, France, France;  ⁴ University of Bordeaux, CNRS, Microscopy Department IBGC, UMR 5095, F-33000 9 Bordeaux, France, France

Discussion

TFK1 is a kinetoplastid specific protein and a mature and maturing BB marker, localized on the transitional fibre. TFK1 is the third component of the transition fibres region, with TbRP2 and CEP164C, in T. brucei. Our high-resolution ultrastructure expansion microscopy data demonstrate that TFK1 is displayed in a typical radial arrangement in the distal appendage matrix, as nine dense points between the molecules of CEP164C. TFK1 is essential for BSF, unlike PCF. Its depletion induces, on one hand, previously undescribed cytokinesis defects by the absence of furrow associated with segregation of BBs similar to that of PCFs (KNKN) and on the other hand, leads to the blockage of abscission during cytokinesis in BSFs