Abstract

In Drug Discovery and Process Monitoring, there is an unmet need for convenient, rapid, and accurate methods for analysing interactions between molecules. Label-free assays are the most widely used method which offers several benefits to the user, including real-time data read-out, quantitative data, and high sensitivity. However, current label-free, optical-based systems like surface plasmon resonance (SPR) and bio-layer interferometry (BLI), suffer from loss of sensitivity with decreasing molecular size, high cost, and complex operating procedures. To address the limitations of optical-based, label-free analysis platforms, HexagonFab has developed a graphene-based sensor system, named Bolt. The sensor measures changes in the electrical properties of a charge-sensitive graphene membrane which are induced by the presence of molecules at the sensor-analyte interface (e.g., proteins, small molecules, or DNA/RNA). The aim of this presentation is to showcase the HexagonFab Bolt as a new user-friendly tool for measuring the affinity of protein binders (antibodies and nanobodies) and spark a conversation to identify more exciting applications for our tool.