Abstract

Understanding the interactions between metabolites and small molecules on one hand, and the proteome on the other, are vital for our understanding of biological phenotypes, metabolism and on drug action. In this lecture, I’ll summarize our efforts in using proteomics combined with systematic perturbation experiments to derive metabolic function principles.I’ll present a new proteomic platform designed to measure thousands of sample at low cost, and its application to generate a proteome for each knock-out strain. Generating such dataset, allowed us to bridge genome, proteome and metabolome on the scale of genome wide genetic perturbation, and I will illustrate on the basis of several examples, on how genome-scale proteomic data opens new avenues for understanding gene function and interactions within biological systems.