Abstract

Background: The ability to detect nucleic acid-based biomarkers from circulating blood is paramount for numerous conditions like cancer where early diagnosis is critical for better patient prognosis. Current nucleic acid detecting techniques are costly, time consuming, require multiple pre-assay sample preparation steps, specialised equipment and complex data interpretation software. 

Aim: Here, we utilize Nanovery nucleic acid biomarker detection kits as diagnostic devices to detect spiked biomarkers in serum, complex media and Nanovery Buffer®. 

Experimental procedures: The nanorobot is assembled based on 4 interchangeable modules namely detector, translator, amplifier and responder. We utilize artificial intelligence to efficiently design our nucleic acid-based detectors against target miRNAs and mutations in mRNA and ctDNA. The remainder 3 modules are tailored to the sample characteristics to ensure maximum sensitivity and selectivity. 

Conclusion: Our Nanorobots can be used across different sample types, from complex media to serum, for the detection of nucleic acid biomarkers.