Objective

Human osteosarcoma is an initial malignant tumor of the bone, which affects children, teenagers and adults in old age. It is the sixth most common type of cancer (Niforou et al., 2006). Recent evidence suggests that NFκB pathways play a role in bone abnormalities The NMI provides research and development services in the fields of life science. By employing our expertise in viral RNAi technology and quantitative imaging, we could demonstrate that mimicking age-related impairment of mitochondrial function may provide a novel model for age-related synaptopathy. Human induced pluripotent stem cells (hiPSCs) from clinically diagnosed individuals or synthetic disease models based on CRISPR/Cas9 genome editing of hiPSCs represent powerful test systems accessible by a broad set of available cellular assays. The CRISPR/Cas9 technology opens up new possibilities for disease-related target knockout, knock-in, or mutagenesis. To complement state-of-the-art research in the field of cancer immunotherapy, we have established a variety of patient-derived 3D microtumour models to reproduce biological tissue complexity, also in co-culture with lymphocytes.