The Hermes WiScan® automated microscope coupled with versatile and fully automated WiSoft®
Athena software enables the development of quantitative, sub-cellular imaging
assays for high-throughput phenotypic profiling.
Here, we present case studies where the Hermes system enabled the acquisition of large datasets to probe cellular responses to chemicals, viral infection and protein-protein interactions with robust and reliable statistics.
In each instance, researchers acquired tens of thousands of
multicolour fluorescence images using the Hermes WiScan® hardware. Working closely with Idea Bio-Medical, they
designed custom-made image analysis packages to automate their quantification
needs.
Researchers identified several novel interactions through their images and analysis:
- Nuclear translocation inhibition of intracellular immune signals by HIV-1 virus
as a requirement for infection;
- Alteration in actin-dependent protein-protein
interactions by bacterial infection leading to the innate immune response;
- Quantified coupling of drug cytotoxicity with alterations in protein
expressions for chemotherapy and epigenetics.
