Objective

 Human induced pluripotent stem cell (hiPSC)-derived sensory neurons may be effectively used for pain research and toxicity testing. The purpose of this study was to evaluate the physiological responses against typical pain-related molecules, temperature change and anti-cancer drug in cultured hiPSC-derived sensory neurons using high-throughpupt multi-electrode array (MEA) system. Firstly, we confirmed humen iPSC-derived sensory neurons expressed typical sensory neural marker Nav1.7, TRPV1, and TRPA1 using immunostaining. Next, we detected the physiological responses to temperature change, capsaicin, menthol, and wasabi by change of spike rate using MEA system.Human iPSC-derived neurons were classified into 27 types depending on physiological responses against 3 compounds. Percentage of the neurons having positive function against capsaicin were high both hiPSC-derived neurons and rat DRG neurons. We also detected the responses to anti-cancer drugs oxaliplation and confirmed the no responses to pain relief asprin in cultured hiPSC-derived sensory neurons. Our results suggested that electrophysiological measurement in cultured hiPSC derived sensory neurons using MEA system are suitable to toxicological assay for pain in peripheral nerves system.