Objective

Yeast is a popular model organism because it is easy to genetically modify and robust to differing environments. Yeast can be studied under aerobic conditions when plated on agar plates. The Singer Instruments ROTOR device pins colonies of yeast, fungi or bacteria onto agar plates in 96, 384 or 1536 plate format and enables to study colonies in high-throughput. Here, different yeast clones were pinned onto a plate resembling a 384-well plate to determine the organism's response to varying oxygen concentrations. These concentrations were generated in the CLARIOstar measurement chamber by its atmospheric control unit (ACU). The microplate reader detected differences in yeast autofluorescence, citrate synthase 2 expression reported by mCherry and a redox-sensitive roGFP2-based probe. RoGFPs are redox sensitive green fluorescent proteins that can be made to respond to specific redox species via the genetic fusion of appropriate redox enzymes.