Objective

The Phosphoinositide 3-kinase (PI3K) pathway has been studied extensively for its role in the development of human cancers.  Tools to detect the regulatory impact of PI3K inhibitors on pathway activation have become essential for drug developers.  Indication of pathway activity may be derived from the detection of phosphorylated pathway members downstream of PI3K. In this study, the levels of phospho-Akt are quantitated in Cisbio’s HTRF Cellular Assay kit.