Objective

The newly developed Acoustic Mass Spectrometry (AcMS) platform utilises the power of Mass Spectrometry with the rapid sample delivery from the Acoustic transducer. Development of assays on this platform requires specific buffer optimisation due to signal supression which can be caused by salts and metal ions present in the system. This makes protein kinases an important target class to explore on the AcMS platform due to the requirement for magnesium ions for activity. In this poster we present the approaches we have used to develop kinase assays with optimised buffers that are compatible with AcMS detection. For a model kinase system we will present data comparing the AcMS system with an existing format that have been used to generate IC50 data, showing the data correlation, and comparing quality and precision. We will also present the key learnings with respect to preferred buffers, detergents and tolerated magnesium levels.