Objective

Cyto-Mine® is the world’s first fully integrated instrument system for high-throughput single cell screening and dispensing, using microfluidic picodroplet technologies. By encapsulating single cells in picolitre volume water-in-oil droplets, Cyto-Mine® enables rapid detection of molecules (e.g. antibodies) secreted by individual cells, followed by selective cloning of single cells based on specific antibody production and titre. The system also allows label-free selection and deposition of single cells into well of 96- and 384-well MTPs - ensuring high fidelity monoclonality assurance (required for FDA-approval of all monoclonal antibodies). Data will be presented showing rapid, ultra-sensitive Ab detection, sorting and dispensing of antibody-secreting cells.