Objective

Since its introduction in the 1970’s the ELISA (enzymelinked immunosorbent assay) remains a very popular technique to assess the presence or quantity of an analyte.1,2 Despite the high utility of this approach and the continued advances to improve sensitivity, most ELISA type assays require washing steps. These steps are time-consuming and tedious or require equipment dedicated to the task.Immunoassays that use SPARCL from Lumigen provide no-wash detection. We have previously shown the performance of the Omega microplate reader in detection of this luminescent assay. Here we show the CLARIOstar is also excellent for detection of SPARCL immunoassays. Furthermore we show that the SPARCL assay can be performed in a 384-well format for improved throughput.