Authors

W M Crozier¹; M Tinti¹; A I Lamond²; M A Ferguson¹;  ¹ Biological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee;  ² Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee

Discussion

Trypanosoma brucei is a highly divergent organism in comparison to commonly studied model organisms and its genome is poorly annotated for protein function. Therefore, we have applied global proteomic analyses to gain further insight into cell cycle protein regulation and protein-protein interactions. Here we report the regulation of 5,033 proteins across the cell-division-cycle of the procyclic form (insect stage) of the parasite. Furthermore, we have analysed the elution profiles of 5,845 proteins across three forms of chromatography, building a high-confidence interaction network of 234 complexes comprising 805 proteins.