Sunday, 4 September 2016 to Wednesday, 7 September 2016
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Poster
82 |
The mRNAs that are increased after heat shock of procyclic-form Trypanosoma brucei include many that are induced during differentiation to mammalian-infective forms |
African trypanosome procyclic forms are routinely cultured at 27°C. Heat shock at 37°C and above inhibits translation, and 1h at 41°C causes sequestration of mRNA in granules. By transcriptome analysis we found that mRNAs that bind to the heat shock regulator ZC3H11 remained in polysomes at 39°C and were protected from sequestration in granules at 41°C. The mRNAs that encode ribosomal proteins were excluded from heat-shock granules. 70 mRNAs moved towards the polysomal fraction at 39°C; many of these are also increased when trypanosomes migrate to the Tsetse salivary glands.
