Abstract

The Chemical Biology and Genome Engineering platform (CBGE) is one of 10 infrastructure platforms at SciLifeLab that together make up SciLifeLab’s national research infrastructure.The Platform consists of three units: The Chemical Biology Consortium Sweden (CBCS) offering support in assay development, small molecule screening, enabling chemistry, and access to small molecule libraries. Additionally, CBCS recently expanded services to include disease and compound profiling using morphological profiling (i.e., cell painting), support for functional precision medicine projects, screening infectious pathogens in biosafety level 2 and 3 laboratories, virtual screening, advanced chemoinformatics and automated patch clamp profiling. The CRISPR Functional Genomics unit (CFG) focus is on massively parallel genetic perturbations, however the unit also performs precision genome engineering in cell lines. Genome-wide, pooled CRISPR KO screens are one of the most requested experimental approaches, and CFG has branched out considerably and has established a variety of other experimental techniques. The Chemical Proteomics unit (ChemProt) supports target deconvolution and MoA elucidation after phenotype-based approaches, support is focused on proteome-wide analyses of compound-treated cells and lysates, with project-tailored MS-based approaches and integrated orthogonal method, in mammalian cells or bacteria. For target-based approaches, the unit offers mapping of the protein binding site (e.g., small molecules, antibodies) and monitoring of conformational changes.