Abstract

FIDA is a microfluidic technology which utilizes the phenomenon of Taylor’s dispersion to obtain absolute size in solution hydrodynamic radius (Rh ) of biomolecules. Here we demonstrate how Fida1 instrument can be employed as an effective tool for characterization of guide RNA (gRNA)- Cas nuclease interactions and as an automated gRNA screening method. The interaction analysis and the screening assay requires only a single, fluorescently labelled RNA strand and consumes only 40 nL of it for each measurement.