Abstract

Synthego has developed automated platforms for synthesizing CRISPR sgRNAs, at both RUO and GMP scale, and for engineering cell lines at scale using the CRISPR-Cas technology. In our gene knockout approach, guide RNAs are multiplexed to produce a high likelihood of gene disruption. Here we show how libraries of gene knockout guide RNAs can be used in an arrayed CRISPR screening approach to identify genes associated with cell survival and death, which could be candidates for molecularly targeted drugs. In addition, we show that cells pre-engineered with gene knockouts are valuable for validation studies when identifying genes associated with cancer drug resistance.