Abstract

Over 70% of researchers cannot reproduce another scientist’s experiments, revealed a survey by Nature. Additionally, Bradbury et al. in 2015 estimated that $1.6 billion was lost on poorly characterized reagents worldwide.
To help improve the quality of Science and data reproducibility globally, Abcam recently introduced its Premium Bioactive Proteins, a new high-quality, off-the-shelf product line.   The Premium Bioactive Protein portfolio consists of intra- and extracellular proteins expressed in mammalian cells, produced under ISO 9001:2015 standards. The proteins are all tag-free, which eliminates the possibility of interference from tags in sensitive assays. Following production and purification, each protein undergoes extensive analytical characterization to assess its purity, using intact mass LCMS, Reverse Phase HPLC, SDS-PAGE and endotoxin testing methodologies. Finally, the potency of each protein is determined using a relevant functional assay. The poster will provide an overview of the protein production process and include detailed analytical and functional characterization data provided for all proteins at the time of release. The communication will also describe analytical criteria established to ensure batch-to-batch consistency. The launch of the Premium Protein commercial range is one of the latest initiatives undertaken by Abcam to improve reagent reproducibility across the drug discovery and development sector.