Nuisance compounds are a significant burden in chemical probe and drug discovery. These bad-acting compounds can interfere with the assay technology and/or produce genuine but undesirable bioactivity. While significant progress has been made to understand and mitigate the effects of nuisance compounds in cell-free systems, there are still many barriers to adequately address nuisance compounds in more complex cell-based assays. This presentation will describe several examples of nuisance compounds encountered in complex biological assays such as Cell Painting and live-cell imaging. Current efforts to address cellular nuisance compounds will be presented.
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