Thought Leader Presentation - Single-cell Multiomic Approaches Reveal High Parameter Correlation of Protein and mRNA Expression in Individual Immune Cells

Time: To be announced
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To be announced

Abstract

Today, as the technologies in our industry mature and advance in complexity, speed to answer, ease of use and sensitivity, the opportunity to use multiple approaches to answer high dimensional biological questions are changing and expanding our knowledge of immunology. This presentation will highlight the combined use of high parameter analytical flow cytometry and advanced single cell multi-omic tools to help define deep functional NK cell subsets following challenge with poly-IC treatment in a mouse model. The approach will consist of the use of sorting defined subsets and fluorescent barcoding, an advanced database of the mouse receptor proteome, a targeted set of RNA probes and an analysis using a divisive tree structure for downstream functional subset analysis.
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