AbstractIn this talk we are going to show the application of multimodal mass spectrometry imaging (MSI) in cancer studies. MSI, because of its ability to discover and map hundreds of tissue biomolecules in one experiment without any prior knowledge, becomes a powerful analytical tool in life science. With recent rapid development in MS instrument, ion source and data analysis method, MSI has been increasingly applied to uncover biomolecular changes associated with cancer diagnosis, progression, and treatment. Funded through Cancer Research UK's Grand Challenge scheme, we’ve been focusing on mapping tumours at unprecedented high-resolution level using multimodal MSI techniques, including widely available matrix-assisted laser desorption ionization (MALDI) MSI, desorption electrospray ionization (DESI) MSI, and home-developed atmospheric pressure laser-sampling MSI with post-ionization ion source. The studies so far on colorectal, pancreatic and breast tumours have shown us that using multimodal MSI, not only key metabolic biomarkers can be identified, but the affected metabolic pathways together with their associated genes are also discovered thereby providing potential targets for novel therapeutics. In addition, spatial distribution info of key biomolecules can benefit the study of heterogeneity of tumours, as well as the separation of tumour phenotypes.